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Imaging: Needs Assessment

To identify the imaging needs of the different groups/users in the department

CURRENT USE

(more than one choice possible)

I routinely use:
Non-fluorescent imaging (Brightfield or Darkfield microscopy)
Fluorescence microscopy (GFP etc.) for fixed cells
Fluorescence microscopy (GFP etc.) for live cells
Single particle fluorescent imaging (SPFI)
Whole organism microscopy (e.g. embryo, root hair...)
Flow cytometry
Bioluminescence imaging
Other (please specify):

AVERAGE TIME SPENT

(more than one choice possible)

Microscopy: I spend 1-2 hours a week
Microscopy: I spend 3-6 hours a week
Microscopy: I have pale skin from living in the darkroom
Flow cytometry: I spend 1-2 hours a week
Flow cytometry: I spend 3-6 hours a week
Flow cytometry: The cytometer and I are <i>one</i>
Image data analysis: I spend 1-2 hours a week
Image data analysis: I spend 3-6 hours a week
Image data analysis: I do nothing but

MOST IMPORTANT PARAMETERS

(more than one choice possible)

The most important parameter(s) to me is (are):
Speed (I image fast processes)
Magnification (I image very small structures)
Signal strength (I am dealing with weak fluorescence)
Longevity (I keep my cells happy for a long time)
Large field of view (I image large structures)
Not sure
Other (please specify):

RECORDING LENGTH

(more than one choice possible)

my recordings usually last around

milliseconds
seconds
minutes
hours
days
(and/or) I use fixed cells (time is not an issue)
Not sure

BRIGHTFIELD MICROSCOPY

i.e. non-fluorescent microscopy (using a halogen light source)

(more than one choice possible)

I don’t use brightfield at all
I use brightfield to find my cells/structures before fluorescent imaging
I use brightfield to show cell/structure outlines for the fluorescent imaging
I use brightfield for stained histology specimens
I use brightfield for time-lapse imaging
I use Phase Contrast (PhaCo)
I use DIC (= Nomarski)
Not sure

TECHNIQUES, INSTRUMENTS, PROGRAMS

more than one choice possible

Of the following, I (or my group) use:

FRET calculations (e.g. for SPFI)
Photobleaching experiments (FRAP)
Flow cytometry
Cell manipulation while imaging (e.g. adding substance under microscope)
Excel (to quantify structures in images)
Photoshop (to process images for publication)
Illustrator (to create graphs and cartoons for publication)
Powerpoint (to create graphs and cartoons for publication)
3D reconstructions
Deconvolution
Other filters to de-haze images (i.e. make them more 'crisp')
Not sure
Other (please specify):

DATA ANALYSIS

(more than one choice possible)

I usually use:
Particle tracking
Time-independent localisation (usually in fixed cells)
Time-dependent localisation (in live cells)
3D reconstruction
Co-localisation studies of two proteins
Co-localisation studies of three proteins
Co-localisation studies of four proteins
Not sure
Other (please specify):

PERSPECTIVE

(more than one choice possible)

In 3-5 years from now, I would most like to use
Multiphoton (Two-photon) confocal microscopy
Spinning disk confocal microscopy
High-speed single particle fluorescence imaging
Time-resolved FRET (FLIM-FRET)
Large field of view non-fluorescent imaging
Large field of view fluorescent imaging
A heavy-duty bioluminescence imager
A Hadron collider
Not sure
Other (please specify):

FLUORESCENT TAGS

(more than one choice possible)

I (my group) use one or several of the following fluorophores:
GFP
EGFP
YFP
CFP
RFP
BFP
Rhodamine
FITC (Fluorescein)
TRITC
Cy3®
Cy5®
Alexa Fluor® 532
Alexa Fluor® 430
BODIPY® 530/550
DAPI
DsRed
Hoechst 33342 & 33258
Fura Red™
Pairs: CFP/YFP
Pairs: FITC/TRITC
Pairs: eGFP/DsRed2
Pairs: CFP/DsRed2
Pairs: BFP/eGFP
Three: DAPI/FITC/TRITC
Four: DAPI/FITC/TRITC/Cy5
Four: DAPI/FITC/TRITC/Alexa Fluor647
Kaede
FlAsh and ReAsh
Quantum dots
Other (please specify):
This poll was created on 2009-11-17 10:06:45 by philippe laissue